A PHOTO

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies:

http://figshare.com/articles/Parallel_genome-scale_loss_of_function_screens_in_216_cancer_cell_lines_for_the_identification_of_context-specific_genetic_dependencies/1019859

"Using a genome-scale, lentivirally delivered shRNA library, we performed massively parallel pooled shRNA screens in 216 cancer cell lines to identify genes that are required for cell proliferation and/or viability. Cell line dependencies on 11,000 genes were interrogated by 5 shRNAs per gene. The proliferation effect of each shRNA in each cell line was assessed by transducing a population of 11M cells with one shRNA-virus per cell and determining the relative enrichment or depletion of each of the 54,000 shRNAs after 16 population doublings using Next Generation Sequencing. All the cell lines were screened using standardized conditions to best assess differential genetic dependencies across cell lines. When combined with genomic characterization of these cell lines, this dataset facilitates the linkage of genetic dependencies with specific cellular contexts (e.g., gene mutations or cell lineage). To enable such comparisons, we developed and provided a bioinformatics tool to identify linear and nonlinear correlations between these features."

#cancer #genome #genetics

A PHOTO

Check out this example of the extra impact your research can have by sharing on figshare: 

http://www.altmetric.com/details.php?&citation_id=2653335

#openresearch #altmetrics

A PHOTO

Drosophila CNS function is robust to wiring variability:

http://figshare.com/articles/Drosophila_CNS_function_is_robust_to_wiring_variability/1185642

#neuroscience #genetics

A PHOTO

Annual sea surface temperature anomalies: 1992 - 2010:

http://figshare.com/articles/Annual_sea_surface_temperature_anomalies_1992_2010/1183470

"Annual mean sea surface temperature (SST) anomaly maps for 1992 - 2010. The anomalies are the annual mean SST minus the 1992 - 2010 mean SST. The underlying data are derived by thermal remote sensing, and are independent of (but highly consistent with) SST information obtained from ocean surface measurements. The remotely sensed SST have been spatio-temporally completed (in-filled) using optimal interpolation, before averaging from daily fields to the annual maps shown here."

#climatechange #oceanography

A VIDEO

brucesterling:

*Martian spacecraft staffers at Indian space control, September 2014

Reblogged from BruceS
A PHOTO

Some thoughts on ‘Trust when it comes to academic research’:

http://figshare.com/blog/Trust_when_it_comes_to_academic_research/134

"In order to be in control of research outputs at a funder, institutional or even researcher level, there needs to be an element of trust with regards to the technologies adopted. At a session on standards for trusted digital repositories, a lot of the debate was around the word ‘trust’ and what that means to different people"

#research #data #RDAPlenary 

A VIDEO

Reconciling DNA replication and transcription in a hyphal organism: Spatial dynamics of transcription complexes in live Streptomyces coelicolor:

http://figshare.com/articles/Reconciling_DNA_replication_and_transcription_in_a_hyphal_organism_Spatial_dynamics_of_transcription_complexes_in_live_Streptomyces_coelicolor/1181785

RpoC-eGFP foci tracking behind the extending hyphal tip. Video of growing S. coelicolor hyphae (LN301; rpoC-egfp) showing the absence of RNAP-eGFP foci at the tip of extending vegetative hyphae. Images taken at 10 min intervals and converted to video using IPLab scientific imaging software version 3.7 (Scanalytics, Inc., Rockville, USA).

#microbiology

A PHOTO

Nice infographic from Taylor and Francis - ‘Mapping car ownership in Great Britain over four decades’: 

http://figshare.com/articles/Mapping_car_ownership_in_Great_Britain_over_four_decades/1177008

A PHOTO

A conceptual diagram of story elements that contributed to tension or conflict in stories about data management, as collected through interviews with researchers:

http://figshare.com/articles/Data_Stories_Map_of_conflicts_in_stories_about_data_management_and_sharing_updated_17_Sept_2014/1172375

#research #data #management